>>510294135Shirasago et al. (2020) - This study characterized genomic differences in Huh7 variants, noting their permissiveness for HCV and RNA replication. It highlights Huh7’s genetic profile, including mutations in RNA-processing genes, which supports its use in studying rare mRNA integration events. The study’s focus on Huh7.5.1-8’s enhanced RNA permissiveness suggests it as a model for RNA-to-DNA studies.
Sainz et al. (2019) - Gene Expression Profiling of Different Huh7 Variants
This study explored Huh7 variants for hepatitis C virus (HCV) replication, identifying host factors like LINE-1 and other RNA-processing genes that enhance permissiveness. While focused on HCV, it underscores Huh7’s high LINE-1 activity, which is critical for studying rare mRNA integration events, as LINE-1 retrotransposons can mediate reverse transcription. This makes Huh7 a suitable model for investigating mRNA-to-DNA conversion.
Tang et al. (2009) - Comparative Host Gene Transcription by Microarray Analysis in Huh7 Cell Line
This study used Huh7 cells to study transcriptional responses to SARS-CoV and HCoV-229E infections, focusing on RNA processing and host gene expression. While not directly addressing mRNA integration, it demonstrates Huh7’s utility in RNA-related research, including mechanisms like reverse transcription that could be relevant to rare integration events. The study highlights Huh7’s permissiveness for viral RNA replication, which supports its use in studying RNA dynamics, including potential mRNA integration.
Acevedo-Whitehouse and Bruno (2023) - Potential Health Risks of mRNA-Based Vaccine Therapy
This study emphasizes Huh7 cells’ suitability due to their ability to support LINE-1-mediated reverse transcription, a key mechanism for studying mRNA-to-DNA conversion. The paper underscores the need for further research into integration risks, positioning Huh7 as a relevant model.