>>513772406 (OP)
1. What you’re actually seeing
The screenshot shows a sequence ending with a stretch of As.
The spike protein gene is RNA, so in the gene sequence, the coding strand uses A, U, C, G (or A, T, C, G in DNA).
A long stretch of As at the end is normal because of the poly-A tail in mRNA, which is required for stability and translation.
2. Why 33 A’s is not mysterious
In coronaviruses, mRNA ends with a poly-A tail. The tail can vary in length depending on how the mRNA is processed.
A stretch of exactly 33 A’s in a particular sequence file is just the sequence included in that reference genome file, not the only biologically possible length.
Different databases may show slightly different lengths of poly-A tails; there’s nothing special about “33” – it’s just what’s in this file.
3. Misunderstanding of gene vs mRNA
The anonymous poster seems to confuse the coding sequence of the spike protein with the mRNA tail.
The spike coding sequence does not “end with 33 A’s” in terms of amino acids. Amino acids are coded by codons, so a stretch of AAA codons codes for lysine (K), not “A” in RNA sense.
4. How this happens in ClustalX
ClustalX shows sequences aligned, and sometimes the software displays trailing A’s or gaps at the end of the alignment because that’s how the reference genome was input.
The “end of file” note is just indicating that the sequence ends in the file, not that 33 A’s are a secret signal.
Conclusion
There is nothing suspicious about 33 A’s at the end of the Wuhan-Hu-1 spike gene file.
This is just a normal poly-A tail in the mRNA, or possibly a quirk of how the reference sequence was saved.
The idea that “exactly 33 A’s” is some hidden code or design is entirely baseless.
8/23/2025, 9:37:27 AM
No.513772406
>>513772446
>>513772467
>>513772823
>>513776677
>>513779001
