42 characters (counting Pyra/Mythra as 1 character and Pokemon Trainer replaced with solo Charizard again)

Newcomers:
Paper Mario (Mario)
Ring Fit Trainer (Ring Fit)
Bandana Dee (Kirby)
Noah & Mio (Xenoblade)
FE shill pick from an unannounced game (Fire Emblem)
Pokemon shill pick from an unannounced game (Pokemon)
Master Chief (Halo)
John Fromsoft (The Duskbloods)