Scientific evidence:

Periodic changes in serum testosterone levels after ejaculation in men
https://pubmed.ncbi.nlm.nih.gov/12506329/

Influence of abstinence and ejaculation-to-analysis delay on semen analysis parameters of suspected infertile men
https://pubmed.ncbi.nlm.nih.gov/7114956/

Improved sperm kinematics in semen samples collected after 2 h versus 4-7 days of ejaculation abstinence
https://pubmed.ncbi.nlm.nih.gov/28531319/

Influence of a short or long abstinence period on semen parameters in the ejaculate of patients with nonobstructive azoospermia
https://pubmed.ncbi.nlm.nih.gov/11532469/

Seasonal changes in breeding activity, testicular size, testosterone concentration and seminal characteristics in rams with long or short breeding season
https://pubmed.ncbi.nlm.nih.gov/6706874/

Shorter abstinence decreases sperm deoxyribonucleic acid fragmentation in ejaculate
https://pubmed.ncbi.nlm.nih.gov/21924714/

Endocrine response to masturbation-induced orgasm in healthy men following a 3-week sexual abstinence
https://pubmed.ncbi.nlm.nih.gov/11760788/

Relationship between sexual satiety and brain androgen receptors
https://pubmed.ncbi.nlm.nih.gov/17268169/

A research on the relationship between ejaculation and serum testosterone level in men
https://pubmed.ncbi.nlm.nih.gov/12659241/